AATCC-100 Fabric Antibacterial Test (Quantitative)
AATCC 100 Anti-bacterial Assessment or Bacterial Resistance Test on textile Material Fabric anti-bacterial test (quantitative)
With the advancement of nanotechnology and the continuous development of antibacterial additives, the antibacterial ability of fabrics, linings, fillers and other fabrics has been valued as an important indicator of product quality. The antibacterial ability of the product is evaluated qualitatively and quantitatively through standardized testing. The evaluation methods commonly used internationally generally refer to AATCC100 (quantitative) and AATCC 147 (qualitative). During the antibacterial test, the simulated environment and conditions include: culture medium concentration, temperature and humidity, pH value and test time to match those worn in real life. clothing environment and conditions.
AATCC-l00Test method
AATCC-100 is a volumetric quantitative analysis method suitable for the evaluation of the antibacterial rate of antibacterial fabrics.
This law was proposed by the AATCC Committee in 1961 and revised in 1965, 1981, 1988, 1993, and 2004. It is one of the most widely used antibacterial test methods in foreign countries.
The principle of this method is: inoculate the test bacteria on the sample to be tested and the comparison sample, add a certain amount of neutralizing solution respectively, shake vigorously to wash out the bacteria, measure the bacterial concentration in the eluate using the dilution plate method, and compare it with the control sample Calculate the percentage reduction of bacteria on the fabric compared to The disadvantages of this method are that there cannot be too many specimens in one test and it takes a long time; for non-dissolving samples, the antibacterial performance cannot be evaluated; the ingredients of the neutralizing solution are not specified in detail; and the bacterial solution is too rich in nutrients. , which is too different from the actual wearing conditions; the container is too large and difficult to operate.
On the basis of learning from domestic and foreign experience, after a large number of experiments and improvements, a systematic quantitative testing method system has been formed, which can meet the needs of testing the antibacterial performance of different antibacterial fabrics. That is, improved AATCC-l00, the key points are as follows:
Change the sample of the AATCC-100 method from a circle with a diameter of 4.8cm to a square with a side length of about 1.8cm, and put it into a 30mL or 50mL conical flask with a lid. Use 0.85% ice-cold saline (0-4°C) instead of AATCC broth to dilute the inoculum. Dilute the bacterial strain from about 108-109cfu/mL to 1×105-2×105cfu/mL to prepare an inoculum liquid. Use 20mL, 0.85% ice-cold physiological saline instead of neutralizing agent to clean the sample [14].
Use the following formula to calculate the antibacterial activity and bactericidal activity of the sample:
Antibacterial rate = (the number of viable bacteria in the blank comparison sample after 18h – the number of viable bacteria in the sample after 18h) / the number of viable bacteria in the blank comparison sample after 18h × 100%
Sterilization rate = (the number of viable bacteria in the blank comparison sample at ‘0’ – the number of viable bacteria in the sample after 18 hours) / the number of viable bacteria in the blank comparison sample at ‘0’ × 100%
This method can conduct antibacterial tests on both dissolving and non-dissolving samples, and the nutrients of the culture medium are suitable for the use conditions of the fabric.
The chemical industry provides customers with fabric antibacterial agents that can pass the AATCC100 test
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